1ZD9
Structure of human ADP-ribosylation factor-like 10B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.731, 72.092, 36.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.050 - 1.700 |
| R-factor | 0.21371 |
| Rwork | 0.212 |
| R-free | 0.24802 |
| Structure solution method | SIRAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.561 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.050 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.046 | 0.225 |
| Number of reflections | 18430 | |
| Completeness [%] | 99.1 | 91.7 |
| Redundancy | 6.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | PEG 4000, Magnesium Chloride, Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
