1ZD0
Crystal structure of Pfu-542154 conserved hypothetical protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-17 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 53.460, 53.460, 85.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.270 - 1.700 |
Rwork | 0.202 |
R-free | 0.25200 |
Structure solution method | SAS |
RMSD bond length | 0.016 |
RMSD bond angle | 1.422 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.03) |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.045 | 0.036 | 0.210 |
Number of reflections | 15870 | ||
Completeness [%] | 98.1 | 99.2 | 85.1 |
Redundancy | 9.5 | 9.8 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | modified microbatch | 7.7 | 293 | Magnesium Acetate and PEG 3350, pH 7.7, modified microbatch, temperature 293K |
1 | modified microbatch | 7.7 | 293 | Magnesium Acetate and PEG 3350, pH 7.7, modified microbatch, temperature 293K |
1 | modified microbatch | 7.7 | 293 | Magnesium Acetate and PEG 3350, pH 7.7, modified microbatch, temperature 293K |