1ZCJ

Crystal structure of 3-hydroxyacyl-CoA dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-18
Detector	MARRESEARCH
Wavelength(s)	1.134
Spacegroup name	P 41 21 2
Unit cell lengths	122.910, 122.910, 58.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.17141
Rwork	0.170
R-free	0.22069
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wdk alpha subunit
RMSD bond length	0.014
RMSD bond angle	1.680
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.900
Number of reflections	37374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	PEG 4000, tri-Na citrate, ammonium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K