1ZCE
X-Ray Crystal Structure of Protein Atu2648 from Agrobacterium tumefaciens. Northeast Structural Genomics Consortium Target AtR33.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.642, 42.791, 43.684 |
| Unit cell angles | 90.00, 113.67, 90.00 |
Refinement procedure
| Resolution | 27.180 - 1.300 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.21100 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.058 | 0.107 |
| Number of reflections | 63550 | |
| <I/σ(I)> | 16.75 | 7.53 |
| Completeness [%] | 95.6 | 98.8 |
| Redundancy | 4.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100 mM MES (pH 6), 40% PEG 20K, 100 mM magnesium acetate, 5 mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
