1ZC4

Crystal structure of the Ral-binding domain of Exo84 in complex with the active RalA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97945
Spacegroup name	P 1 21 1
Unit cell lengths	52.912, 113.389, 70.680
Unit cell angles	90.00, 102.77, 90.00

Refinement procedure

Resolution	43.790 - 2.500
R-factor	0.24
Rwork	0.208
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.480	2.480
Rmerge	0.090	0.444
Number of reflections	28917
Completeness [%]	99.8	99.7
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	18-22% PEG3350, 0.1-0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	18-22% PEG3350, 0.1-0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K