1ZC2
Crystal Structure of plasmid-encoded class C beta-lactamase CMY-2 complexed with citrate molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2004-06-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979565 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.710, 97.100, 103.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.850 - 2.090 |
| R-factor | 0.2284 |
| Rwork | 0.225 |
| R-free | 0.29893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.036 |
| RMSD bond angle | 2.752 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 2.210 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 43482 | |
| Completeness [%] | 98.1 | 90.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | citric acid 0.1M, PEG 6000 30 % w/v, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
