1ZBX

Crystal structure of a Orc1p-Sir1p complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9787, 0.9794, 1.1
Spacegroup name	I 2 2 2
Unit cell lengths	70.000, 137.210, 139.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.200 - 2.500
R-factor	0.2202
Rwork	0.220
R-free	0.26030
Structure solution method	combination of SIRAS, MAD and molecular replacement
Data reduction software	MADNESS
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.200	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.054
Number of reflections	26351
Completeness [%]	94.3	85.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	290	MES, NaCl, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K