1ZBT

Crystal structure of Peptide chain release factor 1 (RF-1) (SMU.1085) from Streptococcus mutans at 2.34 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-21
Detector	ADSC QUANTUM 210
Wavelength(s)	0.99187,0.97960,0.97942
Spacegroup name	P 43 21 2
Unit cell lengths	69.444, 69.444, 188.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.580 - 2.340
R-factor	0.22326
Rwork	0.221
R-free	0.27500
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.255
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.580	28.580	2.470
High resolution limit [Å]	2.340	7.400	2.340
Number of reflections	20155
<I/σ(I)>	8.1	22.4	1.3
Completeness [%]	99.6	94.6	99.8
Redundancy	3.5	3.1	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	13.0% PEG-8000, 0.2M Ca(OAc)2, 20.0% Glycerol, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K