1ZBO
X-Ray Crystal Structure of Protein BPP1347 from Bordetella parapertussis. Northeast Structural Genomics Consortium Target BoR27.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97894 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.015, 101.380, 122.223 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.530 - 2.600 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.29700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.093 | 0.284 |
| Number of reflections | 25763 | |
| <I/σ(I)> | 26.15 | 8.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 180 mM sodium formate, 18% PEG3350, 5 mM DTT. , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
