1ZBF

Crystal structure of B. halodurans RNase H catalytic domain mutant D132N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-17
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 31 2 1
Unit cell lengths	66.822, 66.822, 58.667
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.500
R-factor	0.189
Rwork	0.189
R-free	0.21400
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.056	0.417
Number of reflections	23577
<I/σ(I)>	35.1	2.7
Completeness [%]	96.0	97.7
Redundancy	7.3	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	PEG 2000 monomethyl ether, ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	PEG 2000 monomethyl ether, ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	PEG 2000 monomethyl ether, ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K