1ZB9

Crystal structure of Xylella fastidiosa organic peroxide resistance protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	110
Detector technology	CCD
Collection date	2003-10-05
Detector	MARRESEARCH
Wavelength(s)	1.453
Spacegroup name	P 65 2 2
Unit cell lengths	87.655, 87.655, 160.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	1.880 - 1.800
Rwork	0.196
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	1.880	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	34330
<I/σ(I)>	6.2	2.2
Completeness [%]	99.9	100
Redundancy	10.8	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	293	Peg 4000, Tris-HCl, 5mM t-BOOH, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K