1ZB8
Crystal structure of Xylella fastidiosa organic peroxide resistance protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.453 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 91.697, 91.697, 157.178 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.310 - 2.400 |
| R-factor | 0.19491 |
| Rwork | 0.192 |
| R-free | 0.24371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n2f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.739 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.310 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 15924 | |
| <I/σ(I)> | 10.3 | 2.8 |
| Redundancy | 7.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | Peg 4000, Tris-HCl, 0,29mM t-BOOH, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
