1ZAY

PURINE REPRESSOR-HYPOXANTHINE-MODIFIED-PURF-OPERATOR COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	C 2 2 21
Unit cell lengths	176.449, 95.187, 81.777
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
Rwork	0.180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pnr
RMSD bond length	0.010
RMSD bond angle	1.114
Data reduction software	bioteX
Data scaling software	bioteX
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	17098
<I/σ(I)>	14.2	2.8
Completeness [%]	88.0	75
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		pH 7.5, VAPOR DIFFUSION, HANGING DROP