1ZAW

Ribosomal Protein L10-L12(NTD) Complex, Space Group P212121, Form A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-19
Detector	MARRESEARCH
Wavelength(s)	1.05, 0.9793, 0.9795, 0.95
Spacegroup name	P 21 21 21
Unit cell lengths	84.934, 84.933, 63.939
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.233
Rwork	0.222
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.130
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	24019
Completeness [%]	99.4	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	Hepes, MPD, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K