1ZAA
ZINC FINGER-DNA RECOGNITION: CRYSTAL STRUCTURE OF A ZIF268-DNA COMPLEX AT 2.1 ANGSTROMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 45.400, 56.200, 130.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.100 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.340 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.048 * |
| Total number of observations | 34488 * |
| Number of reflections | 9458 |
| Completeness [%] | 93.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | pH 8.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Zif complex | 1 (mM) | |
| 2 | 1 | drop | 450-750 (mM) | ||
| 3 | 1 | drop | bis-tris-propane-HCl | 125 (mM) | |
| 4 | 1 | reservoir | PEG400 | 0-10 (%) | |
| 5 | 1 | reservoir | 450-650 (mM) | ||
| 6 | 1 | reservoir | bis-tris-propane-HCl | 25 (mM) |
