1ZA7
The crystal structure of salt stable cowpea cholorotic mottle virus at 2.7 angstroms resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 1999-09-28 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 365.480, 374.860, 402.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cwp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.406 |
| Number of reflections | 1258085 | |
| <I/σ(I)> | 12.1 | 1.9 |
| Completeness [%] | 84.6 | 55.9 |
| Redundancy | 3.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.3 | 298 | 0.3M succinate, 4% PEG 8000, pH 3.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 3.30 |
