1ZA3
The crystal structure of the YSd1 Fab bound to DR5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-14 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.92086 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 147.059, 147.059, 144.947 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.350 |
| R-factor | 0.22975 |
| Rwork | 0.224 |
| R-free | 0.28028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FVE variants |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.339 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 26539 | |
| <I/σ(I)> | 8.3 | 4.2 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 292 | 20% PEG 8000, 0.2M MgAcetate, 0.1M NaCacodylate pH 6.2-6.6, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
