1ZA2
Structure of wild-type E. coli Aspartate Transcarbamoylase in the presence of CTP, carbamoyl phosphate at 2.50 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Detector technology | CCD |
| Collection date | 2004-08-22 |
| Detector | ADSC QUANTAM Q315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 121.442, 121.442, 142.547 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tu0 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.068 | 0.447 |
| Number of reflections | 42599 | |
| <I/σ(I)> | 36.7 | 3.8 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 9 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 5.7 | 293 | 40 mM Citric acid, 3 mM sodium azide, 1mM 2-mercaptoethanol, 1mM CTP, 0.2mM EDTA, pH 5.7, MICRODIALYSIS, temperature 293K |
