1Z9Z

Crystal structure of yeast sla1 SH3 domain 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-23
Detector	MARRESEARCH
Wavelength(s)	0.9781
Spacegroup name	P 41 21 2
Unit cell lengths	83.990, 83.990, 50.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.950
R-factor	0.18036
Rwork	0.178
R-free	0.22902
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.350
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	10.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.066
Number of reflections	12025
<I/σ(I)>	23.44
Completeness [%]	99.6	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	ammonium sulphate, sodium citrate, potassium/sodium tartrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K