1Z9X
Human DRP-1 kinase, W305S S308A D40 mutant, crystal form with 3 monomers in the asymmetric unit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.400, 62.990, 150.970 |
| Unit cell angles | 90.00, 110.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.930 |
| R-factor | 0.32956 |
| Rwork | 0.328 |
| R-free | 0.35074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SXO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.892 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.000 |
| High resolution limit [Å] | 3.930 | 3.930 |
| Number of reflections | 9532 | |
| <I/σ(I)> | 15.4 | 5.8 |
| Completeness [%] | 94.1 | 79.7 |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 14 % PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
