1Z9W
Tetrameric structure of apo-7,8-Dihydroneopterin Aldolase from Mycobacterium tuberculosis
Replaces: 1R7KExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | I 4 |
| Unit cell lengths | 72.957, 72.957, 42.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.570 - 2.500 |
| R-factor | 0.18441 |
| Rwork | 0.179 |
| R-free | 0.23429 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nbu |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.261 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.000 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | 0.371 |
| Number of reflections | 3990 | |
| <I/σ(I)> | 31.3 | 7.6 |
| Completeness [%] | 99.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 300 | PEG 4000, SODIUM ACETATE, PH 4.6, pH 7.60, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
