1Z9S

Crystal Structure of the native chaperone:subunit:subunit Caf1M:Caf1:Caf1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-08
Detector	MARRESEARCH
Wavelength(s)	1.01
Spacegroup name	P 21 21 2
Unit cell lengths	69.800, 180.140, 45.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.200
R-factor	0.224
Rwork	0.223
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.360
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	2.000
Number of reflections	28353

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	283	PEG 8000, cacodylate, calcium acetate , pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 283K