1Z9O

1.9 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain in Complex with the Rat ORP1 FFAT Motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0331
Spacegroup name	P 1
Unit cell lengths	50.048, 50.033, 90.287
Unit cell angles	90.01, 90.00, 60.03

Refinement procedure

Resolution	14.650 - 1.900
R-factor	0.21941
Rwork	0.215
R-free	0.26051
Structure solution method	SAD
Starting model (for MR)	Found Se by molecular replacement w/ 1Z9L
RMSD bond length	0.031
RMSD bond angle	2.492
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.949
High resolution limit [Å]	1.900	1.900
Number of reflections	59506
Completeness [%]		100
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	283	PEG 2000 MME, sodium thiocyanate, tris buffer, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 283K
1	VAPOR DIFFUSION, SITTING DROP	7.4	283	PEG 2000 MME, sodium thiocyanate, tris buffer, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 283K
1	VAPOR DIFFUSION, SITTING DROP	7.4	283	PEG 2000 MME, sodium thiocyanate, tris buffer, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 283K