1Z9F

Crystal structure of single stranded DNA-binding protein (TM0604) from Thermotoga maritima at 2.60 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-08
Detector	ADSC QUANTUM 4
Spacegroup name	F 2 2 2
Unit cell lengths	76.589, 83.406, 86.431
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.220 - 2.300
R-factor	0.23054
Rwork	0.223
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sru
RMSD bond length	0.014
RMSD bond angle	1.492
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.220	47.220	2.360
High resolution limit [Å]	2.300	10.290	2.300
Number of reflections	4323
<I/σ(I)>	7.5	13.1	2.3
Completeness [%]	68.0
Redundancy	4.3	4.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	8	293	25.0% MPD, 0.1M TRIS pH 8.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K