1Z91

x-ray crystal structure of apo-OhrRC15S in reduced form: MarR family protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9794
Spacegroup name	P 41 21 2
Unit cell lengths	55.355, 55.355, 77.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.500
R-factor	0.223
Rwork	0.223
R-free	0.30500
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	45.175
High resolution limit [Å]	2.500
Number of reflections	4532

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	30 to 33 % PEG 1450, 0.1 M ammonium acetate (or sodium acetate), and 0.1M sodium acetate, pH 4.5., VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	30 to 33 % PEG 1450, 0.1 M ammonium acetate (or sodium acetate), and 0.1M sodium acetate, pH 4.5., VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	30 to 33 % PEG 1450, 0.1 M ammonium acetate (or sodium acetate), and 0.1M sodium acetate, pH 4.5., VAPOR DIFFUSION, HANGING DROP, temperature 298K