1Z90

X-ray structure of gene product from arabidopsis thaliana at3g03250, a putative UDP-glucose pyrophosphorylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-13
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	C 1 2 1
Unit cell lengths	188.680, 58.863, 89.862
Unit cell angles	90.00, 100.40, 90.00

Refinement procedure

Resolution	50.000 - 1.860
R-factor	0.20319
Rwork	0.201
R-free	0.24900
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.607
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.365	1.900
High resolution limit [Å]	1.863	1.860
Rmerge	0.066	0.395
Number of reflections	79505
<I/σ(I)>	17.377	3.224
Completeness [%]	98.0	89.5
Redundancy	7	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	293	1 mg/ml protein, 30% PEG2K, 0.100M MES/ACETATE, 5% DMSO, vapor diffusion, hanging drop, pH 5.5, temperature 293K
1		5.5	293	1 mg/ml protein, 30% PEG2K, 0.100M MES/ACETATE, 5% DMSO, vapor diffusion, hanging drop, pH 5.5, temperature 293K