1Z8V
The Structure of d(GGCCAATTGG) Complexed with Netropsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8457 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.025, 38.559, 53.203 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.600 - 1.750 |
| Rwork | 0.200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB ENTRY DD0002 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 5724 | |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 98.1 | 96.2 |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 290 | potassium cacodylate, MPD, magnesium chloride, spermine, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | potassium cacodylate | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | spermine | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | magnesium chloride |
