1Z8T
Structure of Mutant Pyrrolidone Carboxyl Peptidase (E192Q) from a Hyperthermophile, Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2000-07-23 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.970, 104.520, 79.050 |
| Unit cell angles | 90.00, 91.67, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| Rwork | 0.209 |
| R-free | 0.24420 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1iof |
| RMSD bond angle | 0.005 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.054 | 0.126 |
| Number of reflections | 32556 | |
| <I/σ(I)> | 17.5 | 4.1 |
| Completeness [%] | 90.6 | 78.9 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
