1Z8F
Guanylate Kinase Double Mutant A58C, T157C from Mycobacterium tuberculosis (Rv1389)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 112.306, 112.306, 112.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.310 - 2.500 |
| R-factor | 0.18314 |
| Rwork | 0.181 |
| R-free | 0.23576 |
| Structure solution method | Difference Fourier Map |
| Starting model (for MR) | 1s4q |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.444 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 8301 | |
| <I/σ(I)> | 35.6 | 5.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2.6M sodium formate, 2% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
