1Z85

Crystal structure of a predicted rna methyltransferase (tm1380) from thermotoga maritima msb8 at 2.12 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-23
Detector	ADSC QUANTUM 210
Wavelength(s)	0.979694,1.019775,0.979478
Spacegroup name	P 21 21 21
Unit cell lengths	65.652, 82.473, 106.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.320 - 2.120
R-factor	0.1735
Rwork	0.171
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.644
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.320	29.320	2.230
High resolution limit [Å]	2.120	6.700	2.120
Number of reflections	33166
<I/σ(I)>	9	17.1	1.5
Completeness [%]	99.5
Redundancy	3.6	3.4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	8	277	1.0M LiCl, 20.0% PEG-6000, 0.1M TRIS pH 8.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K