1Z84

X-ray structure of galt-like protein from arabidopsis thaliana at5g18200

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-15
Detector	APS-1
Spacegroup name	P 21 21 21
Unit cell lengths	62.037, 95.650, 110.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.280 - 1.830
R-factor	0.18833
Rwork	0.186
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1VKV
RMSD bond length	0.017
RMSD bond angle	1.558
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.486	1.900
High resolution limit [Å]	1.834	1.830
Rmerge	0.083	0.386
Number of reflections	57501
<I/σ(I)>	15.249	4.543
Completeness [%]	96.5	71.4
Redundancy	6.9	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	293	10 MG/ML PROTEIN, 20% PEG 2000, 0.2M Sodium Chloride, 0.10M MES-Acetate, vapor diffusion, hanging drop, temperature 293K, pH 5.50