1Z83
Crystal structure of human AK1A in complex with AP5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.194, 46.259, 119.576 |
| Unit cell angles | 90.00, 116.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.17166 |
| Rwork | 0.169 |
| R-free | 0.21832 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.622 |
| Data reduction software | EVAL15 |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.035 | 0.100 |
| Number of reflections | 49395 | |
| <I/σ(I)> | 21.8 | 9.9 |
| Completeness [%] | 95.7 | |
| Redundancy | 7.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | MES, PEG550, Zinksulphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
