1Z82

Crystal structure of glycerol-3-phosphate dehydrogenase (TM0378) from THERMOTOGA MARITIMA at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.891940,0.979245
Spacegroup name	P 1 21 1
Unit cell lengths	65.090, 67.451, 75.597
Unit cell angles	90.00, 113.67, 90.00

Refinement procedure

Resolution	29.810 - 2.000
R-factor	0.16824
Rwork	0.166
R-free	0.21100
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.621
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.810	29.810	2.050
High resolution limit [Å]	2.000	8.940	2.000
Number of reflections	40008
<I/σ(I)>	7.2	19.1	1.3
Completeness [%]	98.4
Redundancy	3.5	3.3	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	8	277	0.2M MgCl2, 35.0% MPD, 0.1M Imidazole pH 8.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K