1Z81

Crystal Structure of cyclophilin from Plasmodium yoelii.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-06
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	64.230, 64.230, 156.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.266
Rwork	0.257
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mzw
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.750	2.750
Number of reflections	9050
Completeness [%]	98.6	100
Redundancy	10	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	Ammonium sulfate, lithium chloride, Tris HCl buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K