1Z7Y
Crystal Structure of the Arabidopsis thaliana O-Acetylserine Sulfhydrylase K46A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-05 |
Detector | BRUKER SMART 6000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 105.856, 105.856, 100.159 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.700 |
Rwork | 0.251 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oas |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 15955 | |
Completeness [%] | 98.5 | 94.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | sodium formate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |