1Z7W
Crystal Structure of O-Acetylserine Sulfhydrylase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-30 |
| Detector | BRUKER SMART 6000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.153, 105.153, 100.133 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| Rwork | 0.186 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oas |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 26988 | |
| Completeness [%] | 92.7 | 88.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | ammonium sulfate, Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
