1Z7U
Crystal Structure of the Putitive Transcriptional Regulator of MarR Family from Enterococcus faecalis V583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-17 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.754, 63.754, 139.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.930 - 2.200 |
| R-factor | 0.21027 |
| Rwork | 0.205 |
| R-free | 0.26126 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.268 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.930 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.095 | 0.482 |
| Number of reflections | 14767 | |
| <I/σ(I)> | 7.6 | 1.8 |
| Completeness [%] | 79.7 | |
| Redundancy | 11.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | BIS-TRIS-PROPANE, potassium sodium tartrate tetrahydrated, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
