1Z7N
ATP Phosphoribosyl transferase (HisZG ATP-PRTase) from Lactococcus lactis with bound PRPP substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-02 |
| Detector | BRANDEIS - B1.2 |
| Wavelength(s) | 0.921 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 151.684, 222.936, 86.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.000 - 3.250 |
| R-factor | 0.291 |
| Rwork | 0.247 |
| R-free | 0.30140 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 3.450 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.123 | 0.402 |
| Number of reflections | 85130 | |
| <I/σ(I)> | 9.52 | 2.28 |
| Completeness [%] | 98.1 | 93.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | PEG 400, Tris-HCL, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
