1Z7M

ATP Phosphoribosyl transferase (HisZG ATP-PRTase) from Lactococcus lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.2124
Spacegroup name	P 21 21 2
Unit cell lengths	151.684, 222.936, 86.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.900
R-factor	0.298
Rwork	0.245
R-free	0.28550
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.410
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.075	0.467
Number of reflections	110115
<I/σ(I)>	15.72	2.09
Completeness [%]	96.7	82.7
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	PEG 400, Tris-HCL, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	PEG 400, Tris-HCL, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	PEG 400, Tris-HCL, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K