1Z7J
Human transthyretin (also called prealbumin) complex with 3, 3',5,5'-tetraiodothyroacetic acid (t4ac)
Replaces: 1KEDExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-22 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.462, 85.858, 65.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.870 - 2.200 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.21700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | DIMER GENERATED FROM PDB ENTRY 2ROX STRUCTURE WITH ONLY PROTEIN ATOMS FROM RESIDUES 10-125 INCLUDED |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.461 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.059 | 0.061 |
| Number of reflections | 11346 | |
| <I/σ(I)> | 15.2777 | 2.44 |
| Completeness [%] | 94.5 | 73.7 |
| Redundancy | 3.45 | 2.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 48% ammonium sulfate, 0.l M phosphate buffer, pH 5.50, VAPOR DIFFUSION, HANGING DROP, temperature 4.0K |
