1Z7F
Crystal structure of 16 base pair RNA duplex containing a C-A mismatch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-02-14 |
Detector | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 43.000, 43.000, 122.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.010 - 2.100 |
R-factor | 0.261 |
Rwork | 0.261 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yrm |
RMSD bond length | 0.004 |
RMSD bond angle | 0.900 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | 0.176 |
Number of reflections | 8972 | |
<I/σ(I)> | 22.11 | 4.59 |
Completeness [%] | 93.8 | 68.7 |
Redundancy | 12.02 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 273 | MPD, sodium cacodylate, spermine, strontium chloride, magnesium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | magnesium chloride | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | spermine | ||
4 | 1 | 1 | strontium chloride | ||
5 | 1 | 1 | magnesium chloride | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | MPD | ||
8 | 1 | 2 | sodium cacodylate | ||
9 | 1 | 2 | strontium chloride |