1Z70

1.15A resolution structure of the formylglycine generating enzyme FGE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-10-26
Detector	MARRESEARCH
Wavelength(s)	0.918
Spacegroup name	P 21 21 2
Unit cell lengths	62.295, 109.765, 43.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.200 - 1.150
R-factor	0.13914
Rwork	0.138
R-free	0.16991
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y1e
RMSD bond length	0.014
RMSD bond angle	1.769
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0009)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.200	1.170
High resolution limit [Å]	1.150	1.150
Number of reflections	93768
<I/σ(I)>	23.9	5.4
Completeness [%]	88.2	80.8
Redundancy		1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1