1Z6Y
Structure Of Human ADP-Ribosylation Factor-Like 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.657, 48.775, 55.905 |
| Unit cell angles | 112.96, 97.69, 90.00 |
Refinement procedure
| Resolution | 50.900 - 2.400 |
| R-factor | 0.2355 |
| Rwork | 0.233 |
| R-free | 0.28277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rrf |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.704 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.900 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.048 | 0.087 |
| Number of reflections | 13917 | |
| <I/σ(I)> | 10.9 | 5 |
| Completeness [%] | 95 | |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | PEG 4000, ammonium sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
