1Z6S
Ribonuclease A- AMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8115 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.330, 32.609, 72.419 |
| Unit cell angles | 90.00, 90.83, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.19502 |
| Rwork | 0.193 |
| R-free | 0.23148 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.456 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 35273 | |
| <I/σ(I)> | 10.4 | 2.8 |
| Completeness [%] | 98.1 | 99.7 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | Sodium citrate, PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
