1Z6Q

Glycogen phosphorylase with inhibitor in the AMP site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-02
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	127.500, 127.500, 115.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.030
R-factor	0.22
Rwork	0.210
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.029
RMSD bond angle	3.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.060
High resolution limit [Å]	2.030	2.030
Number of reflections	60467
Completeness [%]	99.0	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	BES, EDTA, DTT, SPERMDINE, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K