1Z6N
1.5 A Crystal Structure of a Protein of Unknown Function PA1234 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-29 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 49.686, 75.818, 90.999 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.220 - 1.500 |
| R-factor | 0.19058 |
| Rwork | 0.189 |
| R-free | 0.22047 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.224 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.068 | 0.351 |
| Number of reflections | 27153 | |
| <I/σ(I)> | 30.88 | 2.72 |
| Completeness [%] | 97.4 | 83.6 |
| Redundancy | 8.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris, 0.2 M MgCl2, 28% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
