1Z6G

Crystal structure of guanylate kinase from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-06
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.420, 47.240, 118.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.180
R-factor	0.222
Rwork	0.219
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1EX7 and 1LVG
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	13877
Completeness [%]	99.8	100
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	298	ammonium sulfate, hepes buffer, pH 7.4, VAPOR DIFFUSION, temperature 298K