1Z63
Sulfolobus solfataricus SWI2/SNF2 ATPase core in complex with dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.931, 83.737, 106.390 |
| Unit cell angles | 90.00, 109.78, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 52560 | |
| Completeness [%] | 93.1 | 72.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG3350, Magnesiumformate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG3350 | ||
| 2 | 1 | 1 | Magnesiumformate | ||
| 3 | 1 | 2 | PEG3350 | ||
| 4 | 1 | 2 | Magnesiumformate |
