1Z5Y
Crystal Structure Of The Disulfide-Linked Complex Between The N-Terminal Domain Of The Electron Transfer Catalyst DsbD and The Cytochrome c Biogenesis Protein CcmG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 94 |
| Detector technology | CCD |
| Collection date | 2004-10-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90035 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.574, 53.165, 63.833 |
| Unit cell angles | 90.00, 104.88, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.940 |
| Rwork | 0.235 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JZD for nDsbD homology model based on 1KNG for CcmG |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (v1.2) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 26430 | |
| <I/σ(I)> | 19 | 4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | SODIUM ACETATE, MAGNESIUM CHLORIDE, PEG 4000, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
