1Z5W
Crystal Structure of gamma-tubulin bound to GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.190, 76.290, 64.810 |
| Unit cell angles | 90.00, 102.31, 90.00 |
Refinement procedure
| Resolution | 29.910 - 3.000 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1Z5V stripped of ligands |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.140 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.105 | |
| Number of reflections | 8638 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 85.7 | 82 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 277 | Tris, KCl, PEG6000, pH 8.2, VAPOR DIFFUSION, temperature 277K |
